Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
نویسندگان
چکیده
منابع مشابه
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells
CRISPR (clustered, regularly interspaced, short palindromic repeats)/Cas9 (CRISPR-associated protein 9) has become the tool of choice for generating gene knockouts across a variety of species. The ability for efficient gene editing in primary T cells not only represents a valuable research tool to study gene function but also holds great promise for T cell-based immunotherapies, such as next-ge...
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ژورنال
عنوان ژورنال: Cell & Bioscience
سال: 2019
ISSN: 2045-3701
DOI: 10.1186/s13578-019-0304-0